Dualsystems Biotech - Custom Functional Proteomics Services and Products

Dualsystems major focus is the development of products and services in the emerging area of interactive proteomics. Our areas of expertise include genetic screening in yeast, screening for protein-protein interactions, yeast expression systems and construction of cDNA libraries.

With our innovative protein interaction discovery platform, we are able to find novel protein interactions for any kind of target protein.

We offer screening services to academic and industrial customers and we market a range of related products to help our customers improve their in-house screening platforms. Recently, we developed a yeast-based drug profiling system.

As with protein-protein interactions, the identification of drug-protein interactions can be a formidable task. The identification of the target proteins of small molecules with biological activity is one of the key steps in finding new applications for existing drugs.

With our longtime experience of protein interaction screens we are be able to find drug-protein interactions for any kind of protein.

Yeast-based protein interaction screening technology

How a custom screen is performed:

Supply us with the cDNA encoding your protein of interest Select a cDNA library to be screened from our collection.

Part one:

• Bait cloning and verification

part two:

• Stringency adjustments (pilot screen)
• Library screen and selection of interactors
• Analysis of of up to 384 interactors
• Complete report
• Sequences and BLAST results delivered to customer

Why order a custom screen from Dualsystems Biotech?

• Over eight years of experience, hundreds of screens performed
• Fewer than 1 in a 100 screens yield no results
• An extensively optimized system provides high-quality results in a very short time
• Up to 384 interactors from each screen are analyzed, providing complete coverage
• Average number of primary interactors: 70 clones
• More than 75% of interactors are bait-dependent
• Ready-to-use results enable you to immediately start downstream experiments

DUALmembrane system – protein screening technology

The DUALmembrane system is an extremely powerful screening technology aimed at identifying protein-protein interactions of membrane proteins.

By circumventing problems associated with traditional screening systems, the DUALmembrane system is used to directly identify interactions between membrane proteins in their natural setting.

With the DUALmembrane system you can detect interactions between an integral membrane protein and:

• Other integral membrane proteins (e.g. receptor subunits or co-receptors)
• Membrane-associated proteins (e.g. signalling components or members of the sorting machinery)
• Soluble proteins (e.g. adaptors, kinesis and other signalling components)

DUALhunter system – protein screening technology

The DUALhunter system has been designed for use with proteins that are self-activating in the classical yeast two-hybrid system.

The DUALhunter system lets you screen proteins such as transcription factors by taking the protein interaction out of the nucleus into the cytosol of the yeast cell.

DUALhybrid system- protein screening technology

The DUALhybrid system is based on the classical yeast two-hybrid system. With our triple reporter yeast strain, we guarantee stringent screens and highly reliable results. Big pharmaceutical companies and academic customers utilize our DUALhybrid screens since years.

cDNA library construction

We construct cDNA libraries from tissue, cells or RNA. cDNA libraries are constructed with the following parameters:

• Average insert size at least 1.2 kb

The time of construction depends on the source of the RNA, but is usually between six and eight weeks.

You receive the following from us:

• library quality control sheet
• At least 500 µg of purified library plasmid
• Glycerol stocks for later amplification of the library