Think Big in Purification: Sartocube Crossflow Cassette Provides High Performance and Ease of Use for Large-Scale Purification
21 September 2006
The use of crossflow cassettes in pharmaceutical and biopharmaceutical purification processes has grown by leaps and bounds in the past few years. Especially the biopharmaceutical industry is placing new demands in terms of the performance capability of these components. For this reason, the process specialist Sartorius has developed the monolithic crossflow cassette, called Sartocube. This new development combines the ease of use of conventional cassettes with exceptional high-performance flux rates of the proprietary Hydrosart® membrane. The new crossflow cassette thus closes the gap between small-scale laboratory purification and commercial bioprocesssing for large-scale operation. For the first time, Sartocube enables true scalability with identical hydrodynamic conditions and membranes.
The crossflow cassette has been designed for compliance with cGMP and features validated compatibility for the majority of biopharmaceutical purification applications. Sartocube has a minimal residual working volume and maximum flux rates, while offering greater mechanical and chemical stability. An important feature is its silicon sealing with integrated gasket frame, which allows the seal to be exchanged without using any further tools.
The incorporated Hydrosart® ultrafiltration membrane, which has been shown to deliver higher flux rates than other membranes, improves and increases product yields. It features a broad pH range and exceptionally high flux rates. Thanks to its extremely hydrophilic characteristics, protein binding and fouling are virtually zero. Membrane regeneration, storage and depyrogenation can be easily performed using NaOH even at relatively high temperatures. This property makes Hydrosart® ideal for biological applications.
The new Sartocube series meets the high demands of purification of expensive proteins in crossflow applications. By enhancing performance and increasing yield, Sartocube is a valuable scale-up component developed to make the process for the manufacture of new and already established pharmaceuticals even more cost-effective.
