Scil Proteins Services - Protein Refolding, Recombinant Protein Production and Analytics

If you are looking for a partner who can manage your protein projects with the utmost skill, Scil Proteins Services is the right address. The company offers you a service spectrum and professionalism second to none. It combines the knowledge of a life science expert with the speed and creativity of an entrepreneur.

Scil Proteins Services is a partner organisation placing its costumers in the centre of each project. A dedicated project manager in each stage of the project guarantees that the results will meet the expectations of the customer.

Many pharmaceutical, biotech and diagnostic companies have acknowledged the individualized service provided by Scil Proteins.

E. COLI PROTEIN PRODUCTION

Regardless whether tiny amounts or multiple grams of well-characterized proteins are required, Scil Proteins' experience combined with the extraordinary state-of-the-art equipment allows the production of customised proteins with a reasonable expense of time and money.

Scil Proteins Services addresses each detail of a reliable protein production whether it concerns high-cell density fermentation (scale of 100l; OD600 >140), cost-effective protein refolding, purification procedures, or analytics.

PROTEIN EXPRESSION SYSTEMS

Scil Proteins Services develops and establishes E. coli protein expression systems, which are based on a set of proprietary plasmids and patent free E. coli strains. The fermentations will be carried out in an antibiotic-free environment without any admission of high plasmid stability and expressions yields. These state-of-the-art expression systems fulfil the regulatory requirements and are free of cross-licenses. Customer friendly license conditions make these vector systems attractive for each project whether big or small in size.

Scil Proteins Services also constructs or optimises expression systems according to costumer's requirements. Using its know-how in molecular biology and microbiology, the company has frequently succeeded in switching a non-producer strain into a reliable high producer strain.

PROTEIN REFOLDING AND PROTEIN PURIFICATION

Scil Proteins Services has an outstanding expertise of in vitro protein refolding and of developing industrial and laboratory processes for protein purification. Starting with inactive inclusion bodies the company has reactivated many proteins of different classes by sophisticated but often simple to handle protocols. Examples are growth factors, hormones, kinases, viral proteins, proteases, and enzymes, which have been, produced for diagnostic, pharmaceutical, and biotech companies. Further, a broad range of experience is available for proteins such as G-CSF, tPA, insulin, antibody Fab-fragments, single chain antibodies (scFv), human NGF, BMP, proNGF, proBMP, and the Myeloma Inhibitory Activity (MIA) protein.

PROTEIN ANALYTICS

The company´s protein quality department is known for its meticulous protein analysis using HPLC (rp; size exclusion, ion exchange), spectroscopy (UV-vis, fluorescence, CD) and the Biacore instrument. The Threshold™ system is employed for the determination of DNA and host-cell impurities. The determination of the endotoxin content, ELISA measurements and enzyme assays are part of the department's routine analysis. The analytical package is completed with the development, establishment or performance of in vitro bioactivity assays. Scil Proteins measures cell vitality, cell migration, invasion and differentiation, the ability of proliferation as well as the induction of cellular enzyme activities.

PROCESS DEVELOPMENT OF RECOMBINANT PROTEINS

Scil Proteins Services develops complete production processes in E. coli and optimises single steps thereof. The development of a production process consists of several phases or modules (see column at the right hand). Each module can be realized sequentially but to keep timelines short, the company prefers to develop these steps in parallel, thereby being able to perform the integration of each module into a production scheme as early as possible.

Production processes, developed by Scil Proteins Services and endorsed with a comprehensive set of SOP masters and development reports will be transferred to a partner organisation. These are contract-manufacturing organisations (CMO) working in compliance to GMP standard. National and/or international regulatory authorities have successfully inspected the partners.

On demand Scil Proteins Services takes over the complete responsibility of protein supply for preclinical or clinical studies. In such cases, the company composes a team of internal and external project managers forming a network of excellence.

Scil Proteins would appreciate to provide you with further information or a non-committal quotation. Please contact: