| Date: | 5 Feb 2007 |
| White Paper: | Invitrogen's LanthaScreen TR-FRET Protein Kinase C Assay Using the PHERAstar |
| Abstract: |
BMG LABTECH is a leader in microplate reader technology. Invitrogen's PKC LanthaScreenTM assay has been performed on the PHERAstar microplate reader. Specifically, ten PKC isoenzymes were found to have similar affinities (EC50) for their substrates as reported elsewhere in the literature. The PHERAstar, a multifunctional HTS machine that can read in all detection modes (fluorescence intensity, TRF, fluorescence polarization, luminescence, and absorbance) and can read two emitted wavelengths at once, performed extremely well with this assay as determined by both high signal to noise ratios and high Z'-values. For more information, download this free whitepaper. |