Protected: Mimotopes Custom Peptide Synthesis and Library Technology for Screening
Mimotopes offers high-quality peptide synthesis for the life-sciences industry. It has also created a specialist library technology for the screening of peptides in large numbers.
With more than 20 years of experience in peptide production, Mimotopes specialises in structures of more than 50 amino acids, as well as modifications such as conjugation, cyclisation, biotinylation, and phosphorylation.
Peptide synthesis and modification for the biopharmaceutical industry
Mimotopes synthesises custom peptides to suit the customer’s needs, choosing from a wide range of modification options. Some of the most common requests include residues such as phosphoamino acids, nonradioactive isotope labelling, and branded peptides.
The company also offers a consultation service throughout the ordering process, providing advice on sequence and specification feasibility to produce a more stable, economical, or soluble solution.
Each peptide is processed in an automated synthesiser using a mild 9-fluorenylmethyloxycarbonyl (fmoc) chemistry method. Poor coupling efficiencies are also minimised by using preformed secondary-structure-disrupting dipeptides, pseudoprolines1. Sequence reliability and consistency is ensured by the computer-controlled assembly method.
Peptide fractions are purified and routinely analysed using high-performance liquid chromatography (HPLC) and electrospray mass spectrometry (ESMS). The ESMS technique was specifically developed to prevent breakdown when analysing fragile biomolecules.
Once a peptide is made, Mimotopes also performs immunological services such as epitiope mapping, antipeptide antibody generation, and labelling. The company strives to ensure precice, high-quality products and analytical data.
Common peptide modifications, biotinylation, phosphorylation, and cyclisation
One of the most common peptide modifications is biotinylation. This consists of the addition of a biotin group to a peptide for immobilisation, which allows for the exploitation of biotin-avidin interaction. Mimotopes can immobilise peptides into an avidin-coated plastic or gel, which allows the binding to be measured or used as an absorbent.
Mimotopes also produces phosphopeptides from phosphoamino acids and has extensive experience in affinity-purifying anti-phosphopeptide sera.
In addition, the company offers cyclisation of peptides, which restrains conformations and improves usability. Mimotopes routinely carries out the formation of disulphide bridges and is experienced in the cyclic structures of peptides with two cysteine residues or more. The highest purity offered for these is >80%.
Large-scale synthesis for screening unpurified peptides in vaccine development
Mimotopes’ PepSets library is used for screening large numbers of unpurified custom peptides for a fast and economical service. Each set contains around 96 peptides, and 100 to 1,000 PepSets can be typically supplied within four weeks. Larger sets of around 10,000 are also available. Despite the size of the order, all peptides are supplied at the same time for simultaneous screening in consistent conditions.
PepSets are commonly used for T-cell and B-cell linear epitope mapping, but it can also be used for vaccine development and peptide analogue structure-activity relationship (SAR) studies. A wide variety of product value and flexibility are available to help economically answer vital questions about vaccines, drugs, or diagnostic development research.
One potential use of PepSets is for preliminary screening of antipeptide antibody sites in a disease model. This can help avoid expensive trial and error in vaccine development by predicting the most suitable peptide in the library. Similarly, peptides recognised for immune sera can be identified for immunodiagnostic testing.
Manufacturing and purchasing a PepSet is low-cost due to Mimotopes’ solid-phase technology, as well as simultaneous synthesis in a 96-well array format. This method also allows for faster production and testing. Clients only pay for what they need, with Mimotopes’ synthesis technology allowing customers to choose an appropriate scale and reduce excess.
Documentation and packaging of biopharmaceutical product
Each peptide produced by Mimotopes is provided with a certificate of analysis (C of A). This outlines the quantity and purity of the peptide, as well as information on the ESMS data, the solubility, HPLC profile, and a results interpretation guide.
Packed under dry nitrogen gas, Mimotopes’ peptides are protected from degradation during shipment. A guide to handling the product is provided, which advises on storage conditions upon delivery. In addition, Mimotopes’ support group is available to discuss peptide-based research and access the publications database.
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