A. Menarini Industrie Farmaceutiche Riunite has patented a method for generating a massively parallel sequencing library. The process involves providing a primary WGA DNA library, performing PCR cycles with specific primers, and amplifying the resulting DNA libraries. This innovation allows for efficient and accurate sequencing of DNA samples. GlobalData’s report on A. Menarini Industrie Farmaceutiche Riunite gives a 360-degree view of the company including its patenting strategy. Buy the report here.

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According to GlobalData’s company profile on A. Menarini Industrie Farmaceutiche Riunite, Cancer treatment biomarkers was a key innovation area identified from patents. A. Menarini Industrie Farmaceutiche Riunite's grant share as of January 2024 was 52%. Grant share is based on the ratio of number of grants to total number of patents.

Method of generating massively parallel sequencing library

Source: United States Patent and Trademark Office (USPTO). Credit: A. Menarini Industrie Farmaceutiche Riunite Srl

A recently granted patent (Publication Number: US11859249B2) discloses a method for generating a massively parallel sequencing library. The method involves providing a primary whole genome amplification (WGA) DNA library with specific sections, performing PCR cycles using different primers to extend the DNA library, and amplifying the extended libraries using additional primers. The process includes steps for purifying the DNA libraries at various stages to ensure high-quality sequencing results. The method utilizes specific sequencing adapters and barcodes for accurate sequencing and identification of DNA fragments.

Furthermore, the patent claims specify the inclusion of read sequencing primer sequences and sequencing barcodes in the primers used during the PCR cycles. The method also allows for the use of different universal sequence adapters, such as DRS-WGA or MALBAC adapters, for the WGA DNA library. Specific sequences for these adapters are provided in the claims. Additionally, the middle sequence section of the DNA library can include flanking intermediate sections for further customization and optimization of the sequencing process. Overall, the patented method offers a detailed and structured approach to generating massively parallel sequencing libraries, ensuring efficient and accurate sequencing results for various applications in genomics research and diagnostics.

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GlobalData Patent Analytics tracks bibliographic data, legal events data, point in time patent ownerships, and backward and forward citations from global patenting offices. Textual analysis and official patent classifications are used to group patents into key thematic areas and link them to specific companies