Evotec has filed a patent for recombinant expression vectors combining mCMV enhancer and huCMV intron A sequences for inducible protein expression. The vectors include PiggyBac ITRs for genomic integration and a method for selecting stable production cell lines. Mammalian host cells are also disclosed for protein production. GlobalData’s report on Evotec gives a 360-degree view of the company including its patenting strategy. Buy the report here.
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According to GlobalData’s company profile on Evotec, NSAID cancer drugs was a key innovation area identified from patents. Evotec's grant share as of January 2024 was 39%. Grant share is based on the ratio of number of grants to total number of patents.
Recombinant expression vector for protein production in mammalian cells
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A recently filed patent (Publication Number: US20240035037A1) discloses a recombinant expression vector designed for efficient protein production in mammalian host cells. The vector comprises multiple components, including PiggyBac ITR sequences, expression cassettes with control sequences containing mCMV enhancer elements and CMV promoters, open reading frames encoding proteins of interest, polyadenylation sites, and selectable markers. The vector also includes a third expression cassette with an optional inducible promoter and a second protein of interest. The use of a weak constitutive promoter, such as a deleted SV40 promoter, and specific selectable markers like glutamine synthetase are highlighted in the claims.
Furthermore, the patent describes the application of the recombinant expression vector in cultured mammalian host cells, particularly CHO cells. The method outlined involves culturing the host cells in an aqueous medium under physiological conditions to express the protein of interest and subsequently recovering the protein from the medium. Notably, the vector's design allows for the incorporation of TetO sequences in the control sequences, enabling regulation of protein expression through the presence of tetracycline or its analogs in the culture medium. This derepression mechanism ensures controlled and efficient protein production in the host cells, enhancing the utility of the vector for biotechnological applications.
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GlobalData Patent Analytics tracks bibliographic data, legal events data, point in time patent ownerships, and backward and forward citations from global patenting offices. Textual analysis and official patent classifications are used to group patents into key thematic areas and link them to specific companies
