Nautilus Biotechnology has filed a patent for methods and systems to identify proteins within a sample. The invention involves using a panel of antibodies that are not specific to a single protein or family of proteins. The binding properties of the antibodies are determined, and the protein is exposed to the panel of antibodies. Antibodies that bind to the protein are identified, and the protein’s identity is determined using deconvolution methods based on the known binding properties of the antibodies. The patent claim outlines a method for assaying proteins by immobilizing them at unique spatial addresses and exposing them to different affinity reagents. The pattern of binding of the affinity reagents is used to identify individual proteins. GlobalData’s report on Nautilus Biotechnology gives a 360-degree view of the company including its patenting strategy. Buy the report here.
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According to GlobalData’s company profile on Nautilus Biotechnology, Next-generation sequencing was a key innovation area identified from patents. Nautilus Biotechnology's grant share as of September 2023 was 21%. Grant share is based on the ratio of number of grants to total number of patents.
A method of identifying proteins using affinity reagents
A recently filed patent (Publication Number: US20230296596A1) describes a method for assaying proteins. The method involves providing a plurality of different proteins that are immobilized at unique spatial addresses. These immobilized proteins are then exposed to different affinity reagents, which bind to two or more different proteins. Each affinity reagent has a known degree of binding non-specificity for the different proteins. By determining the binding patterns of the affinity reagents at the unique spatial addresses, the method can identify individual proteins.
The patent claims that the quantity of different proteins identified using this method is greater than the quantity of different affinity reagents used. Additionally, the quantity of different affinity reagents is specified to be less than 500, while the quantity of different proteins identified is at least 1000.
The unique spatial addresses can be on a substrate surface or can be beads. The individual proteins can be immobilized using nucleic acids. The affinity reagents used in the method can include fluorescent tags, monoclonal antibodies, antibody fragments, aptamers, or reagents that bind to amino acids with post-translational modifications.
The identification of individual proteins can be achieved through various algorithms running on a computer system, such as satisfaction problems, expectation maximization approaches, Bayesian inference algorithms, or machine learning algorithms.
The different affinity reagents can include reagents that recognize several different epitopes or a family of epitopes present in multiple proteins.
In the case where the plurality of different proteins is derived from a biological source, the method includes removing high abundance proteins from the sample before immobilizing the proteins at the unique spatial addresses.
Overall, this patent describes a method for assaying proteins that allows for the identification of a large number of different proteins using a smaller number of affinity reagents. The method can be applied to various protein analysis applications, including those derived from biological sources.
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GlobalData Patent Analytics tracks bibliographic data, legal events data, point in time patent ownerships, and backward and forward citations from global patenting offices. Textual analysis and official patent classifications are used to group patents into key thematic areas and link them to specific companies
