Singular Genomics Systems. has been granted a patent for a method of amplifying single-stranded polynucleotides. The method involves hybridizing primer sequences to a solid support, circularizing the polynucleotide, and generating an amplification product using polymerase, with specific blocking features to control extension. GlobalData’s report on Singular Genomics Systems gives a 360-degree view of the company including its patenting strategy. Buy the report here.
Smarter leaders trust GlobalData
According to GlobalData’s company profile on Singular Genomics Systems, DNA polymerase compositions was a key innovation area identified from patents. Singular Genomics Systems's grant share as of July 2024 was 30%. Grant share is based on the ratio of number of grants to total number of patents.
Nucleic acid amplification methods using circular polynucleotides
The granted patent US12071665B2 outlines a method for amplifying single-stranded polynucleotides using a solid support. The process begins by contacting the solid support with a single-stranded polynucleotide that contains specific primer binding sequences. These sequences hybridize to a splint oligonucleotide attached to the solid support. The method involves extending the first primer binding sequence and ligating it to the second primer binding sequence to create a circular polynucleotide. Subsequently, a polymerase extends the splint oligonucleotide to produce an amplification product that is a complement of the circular polynucleotide. This amplification product is then hybridized to a second oligonucleotide on the solid support, which features a blocking group at its 3' end to prevent further polymerase extension.
Further claims in the patent detail variations of the method, including the use of different types of solid supports, such as beads or arrays of wells, and the incorporation of additional primer binding sequences. The patent also specifies the use of strand-displacing polymerases, such as phi29 polymerase, and outlines conditions for the extension process. Notably, the methods allow for the generation of multiple tethered amplification products and include provisions for sequencing these products. The claims emphasize the versatility of the solid support, which can feature a patterned surface or a random distribution of oligonucleotides, enhancing the potential applications in molecular biology and genetic research.
To know more about GlobalData’s detailed insights on Singular Genomics Systems, buy the report here.
Data Insights
From
The gold standard of business intelligence.
Blending expert knowledge with cutting-edge technology, GlobalData’s unrivalled proprietary data will enable you to decode what’s happening in your market. You can make better informed decisions and gain a future-proof advantage over your competitors.
GlobalData, the leading provider of industry intelligence, provided the underlying data, research, and analysis used to produce this article.
GlobalData Patent Analytics tracks bibliographic data, legal events data, point in time patent ownerships, and backward and forward citations from global patenting offices. Textual analysis and official patent classifications are used to group patents into key thematic areas and link them to specific companies
