The Bio Image Intelligent Quantifier (IQ) is a software system for analyzing scanned images of 1-D and 2-D electrophoresis separations, blots, and colonies/plaques. Using the IQ software you can analyze gel files obtained from protein, DNA, RNA, or blot samples and report the data.

General Features

  • Analyze protein, DNA, RNA, carbohydrate, colony & blot samples
  • Analyze wet gels, dry gels, autorads, blots or photographs
  • Import TIFF, JPEG, or BMP files
  • Display intensity in optical density (OD)
  • Name an individual band, spot, cell, or colony
  • Illustrate images with circles, arrows, boxes, and text; combine an image with text for publication-quality work
  • Generate band, spot, colony and blot data analysis reports
  • Export images and/or analysis in standard TIFF, JPEG, Postscript®, and ASCII formats
  • Comprehensive on-line User’s Manual

1-D Electrophoresis Analysis

  • Automatically detect lanes and find bands
  • Find irregularly shaped bands, i.e., biphasic bands, “smiling bands,” or saturated bands that are substantially wider than the rest of the lane
  • Vary band detection sensitivity to optimize performance to gel characteristics
  • Quantify bands with actual band boundaries based on inflection point analysis
  • User-specified Logarithmic, Linear, or Reciprocal methods of interpolation for standards
  • Side-by-side lane profile and band size/quantification comparisons

2-D Electrophoresis Analysis

  • Automatically identify spots in a user-defined area
  • Analyze very complex (10,000 spots) gels
  • Quantify spots using 36 radians per spot for accurate and reproducible quantification data
  • Local background for each spot
  • Logarithmic or Linear interpolation of Mw and pI standards

Dot/Slot Blot Analysis

  • Dot and Slot Blot samples from 1 row by 1 column, up to 52 rows by 52 columns
  • Automatic quantification of individual blot cells
  • Automatic or manual background
  • Normalize cells to a specified row, column, or cell
  • Concentration standards can be assigned to user-selected cells


  • Count colonies in a user-defined area
  • Analyze very complex (10,000 colonies) samples
  • Determine local background for each colony