Caribou Biosciences has filed a patent for DNA-guided CRISPR systems and polynucleotides for use with CRISPR systems. The patent also includes methods of using these polynucleotides and DNA-guided CRISPR systems. The claim specifically describes a set of two Class 2 Type II CRISPR polynucleotides forming a dual guide Class 2 Type II polynucleotide, which includes specific regions and structures necessary for binding with a Cas9 protein. GlobalData’s report on Caribou Biosciences gives a 360-degree view of the company including its patenting strategy. Buy the report here.

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According to GlobalData’s company profile on Caribou Biosciences, CRISPR genome editing was a key innovation area identified from patents. Caribou Biosciences's grant share as of June 2023 was 1%. Grant share is based on the ratio of number of grants to total number of patents.

The patent is filed for a dual guide crispr system

Source: United States Patent and Trademark Office(USPTO). Credit: Caribou Biosciences Inc

A recently filed patent (Publication Number: US20230193324A1) describes a set of two Class 2 Type II CRISPR polynucleotides that form a dual guide Class 2 Type II polynucleotide. The first polynucleotide contains a targeting region with ribonucleic acid (RNA) and an adjacent activating region, while the second polynucleotide contains an activating region that is complementary to the first polynucleotide's activating region. The activating regions of both polynucleotides form a dual guide activating region consisting of a mixture of RNA and deoxyribonucleic acid (DNA). This activating region is capable of binding with a Cas9 protein. The dual guide polynucleotide also includes an activating duplex region with a lower duplex region adjacent to the targeting region and an upper duplex region, with a bulge located between them. The second polynucleotide contains a nexus 3' to the lower duplex region and at least one hairpin 3' to the nexus.

The patent also describes a Class 2 Type II CRISPR complex that includes the dual guide polynucleotide and a Cas9 protein. Additionally, it mentions a cell that contains this CRISPR complex, as well as an isolated cell with the same complex. Furthermore, the patent discusses a Class 2 Type II CRISPR system that consists of the dual guide polynucleotide and a donor polynucleotide.

The patent also outlines a method for modifying a target nucleic acid molecule using the dual guide polynucleotide and a Cas9 protein. This method involves contacting the target nucleic acid molecule with the dual guide polynucleotide and the Cas9 protein, resulting in cleavage of the target nucleic acid molecule. The target nucleic acid molecule can be DNA or RNA, and the contacting can occur in vitro, in a cell, or in an isolated cell. The method also includes the use of a polynucleotide encoding the Cas9 protein and the introduction of a donor polynucleotide. The polynucleotide and the Cas9 protein can form a complex prior to introduction into the cell. Various techniques such as lipofection, electroporation, and microinjection can be used to introduce the polynucleotide into the cell.

Overall, this patent describes a novel dual guide Class 2 Type II CRISPR polynucleotide and its applications in modifying target nucleic acid molecules. The invention has potential implications in genetic engineering and gene editing research.

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GlobalData, the leading provider of industry intelligence, provided the underlying data, research, and analysis used to produce this article.

GlobalData Patent Analytics tracks bibliographic data, legal events data, point in time patent ownerships, and backward and forward citations from global patenting offices. Textual analysis and official patent classifications are used to group patents into key thematic areas and link them to specific companies