Enzo Biochem‘s patent involves methods and compositions for detecting and quantifying chemically modified compounds in samples. The process includes isolating compounds that have gained functional groups from those that have not, using specific techniques like precipitation with charged metal ions and solvents. GlobalData’s report on Enzo Biochem gives a 360-degree view of the company including its patenting strategy. Buy the report here.

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According to GlobalData’s company profile on Enzo Biochem, Cancer treatment biomarkers was a key innovation area identified from patents. Enzo Biochem's grant share as of January 2024 was 54%. Grant share is based on the ratio of number of grants to total number of patents.

Method for detecting enzymes adding functional groups to compounds

Source: United States Patent and Trademark Office (USPTO). Credit: Enzo Biochem Inc

A recently granted patent (Publication Number: US11874281B2) discloses a method for detecting or quantifying an enzyme capable of adding a functional group to a compound in a sample. The method involves providing a compound directly covalently bound to a non-radioactive signaling moiety, a sample suspected of containing the enzyme, a source of the functional group, and means to separate the compound that has gained the functional group. By forming a mixture of the compound, sample, and functional group source, and incubating it under suitable conditions, the compound gaining the functional group is isolated by precipitating it with a charged metal ion and a solvent like ethanol or methanol. The resolubilized compound is then detected or quantified by the non-radioactive signaling moiety.

Furthermore, the method includes the use of a normalizing reagent to promote precipitation of the compound that has gained the functional group, along with specific reagents like barium acetate and EDTA for precipitation and resolubilization, respectively. The non-radioactive signaling moiety can be a variety of compounds like fluorescent, phosphorescent, chemiluminescent, or chromogenic compounds. Additionally, the method allows for blocking moieties on the compound that may interfere with gaining the functional group and offers the option of detecting or quantifying the signaling moiety directly or indirectly. Overall, this patented method provides a comprehensive approach to detecting and quantifying enzymes capable of modifying compounds in a sample, offering a precise and efficient technique for research and diagnostic applications.

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GlobalData Patent Analytics tracks bibliographic data, legal events data, point in time patent ownerships, and backward and forward citations from global patenting offices. Textual analysis and official patent classifications are used to group patents into key thematic areas and link them to specific companies