Zhejiang Huahai Pharmaceutical has patented a method for preparing pregabalin using a biological enzyme process. The method involves producing specific compounds through enzyme reactions and configuration inversions, resulting in the final product. GlobalData’s report on Zhejiang Huahai Pharmaceutical gives a 360-degree view of the company including its patenting strategy. Buy the report here.

Smarter leaders trust GlobalData

According to GlobalData’s company profile on Zhejiang Huahai Pharmaceutical, Cancer treatment biomarkers was a key innovation area identified from patents. Zhejiang Huahai Pharmaceutical's grant share as of April 2024 was 39%. Grant share is based on the ratio of number of grants to total number of patents.

Method for preparing pregabalin using biological enzymes and isomerase

Source: United States Patent and Trademark Office (USPTO). Credit: Zhejiang Huahai Pharmaceutical Co Ltd

A recently granted patent (Publication Number: US11919835B2) discloses a method for preparing pregabalin, a pharmaceutical compound, through a series of specific reactions involving biological enzymes and isomerases. The method involves reacting a compound of formula (I) with a biological enzyme in a first solvent to produce a target compound of formula (III) and a by-product of formula (II). The by-product is then reacted with an isomerase in a second solvent to undergo a configuration inversion, resulting in a compound of formula (IV), which is further reacted with the biological enzyme in a third solvent to yield the target compound of formula (III).

The patent claims specify various aspects of the method, including the type of hydrolase used (e.g., lipase), the source of lipase (e.g., mold lipase, yeast lipase, bacterial lipase), the type of isomerase employed (e.g., epimerase), and the form of enzymes utilized (e.g., immobilized enzyme particles, enzyme powder, cells). Additionally, the patent outlines specific conditions such as mass ratios of reactants, solvent compositions, temperature ranges, and reaction times. The method also includes an extraction step using a fourth organic solvent to remove the by-product before further reactions. Overall, the patent provides a detailed and systematic approach to synthesizing pregabalin, highlighting the importance of enzyme-catalyzed reactions in pharmaceutical manufacturing processes.

To know more about GlobalData’s detailed insights on Zhejiang Huahai Pharmaceutical, buy the report here.

Data Insights


The gold standard of business intelligence.

Blending expert knowledge with cutting-edge technology, GlobalData’s unrivalled proprietary data will enable you to decode what’s happening in your market. You can make better informed decisions and gain a future-proof advantage over your competitors.


GlobalData, the leading provider of industry intelligence, provided the underlying data, research, and analysis used to produce this article.

GlobalData Patent Analytics tracks bibliographic data, legal events data, point in time patent ownerships, and backward and forward citations from global patenting offices. Textual analysis and official patent classifications are used to group patents into key thematic areas and link them to specific companies