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Fluorescence Intensity Transcreener® assay used to detect ADP on the CLARIOstar®

The Transcreener ADP fluorescent intensity (FI) Assay from BellBrook Labs results in a fluorescent intensity output that can be used to quantify enzyme activity.

Any enzyme reaction that creates ADP as a byproduct can be monitored that way, allowing determining activities of e.g. protein, lipid, and carbohydrate kinases, ATPases, DNA helicases, carboxylases and glutamine synthetase.

The Transcreener ADP2 Assay is a simple one-step homogenous detection assay and is flexible with regard to ATP concentration (0.1μM to 100μM ATP). The assay was performed on the CLARIOstar multimode microplate reader from BMG LABTECH.

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