Are you looking to determine the PH-dependent optical rotation of amino acids? This application report ‘Amino Acids – Optically Active Zwitterions’ will help you to understand how you can test the quality of amino acid in your sample.
The importance of the biopharmaceutical industry continues to grow, with recombinant protein production becoming one of the main routes used to deliver therapeutic agents on a commercial scale. Typical production processes use fermentation techniques to build up peptides and proteins from the simpler starting blocks of amino acids. These are either produced in-cells or synthesised in a cell-free process. Both pathways require the addition of the base amino acids as raw ingredients, as some base amino acids cannot be produced by cells themselves.
The quality control of these basic building blocks is crucial to the efficiency of the process and quality of the final proteins produced. One of the techniques of choice for testing the optical purity of amino acids is to use a polarimeter for measuring optical rotation.
Anton Paar offers the most accurate and intuitive polarimeters on the market, and these are fully compliant with the requirements of the international pharmacopoeias including the stricter requirements of the recently revised USP <781>.
Download the application note or request a demonstration now to see first-hand how these units can help your quality control. Download application report here.
Find out more by visiting our website or making an enquiry via the form on this page to request a demonstration.