The Chinese hamster ovary (CHO) cell line is widely used for the production of recombinant proteins due to its high growth capacity and productivity, as well as other cell lines derived later than CHO.

Adapting cell culture media for each specific cell line is a key to exploit these features for cost-effective and fast product generation.

Media supplementation is generally addressed by means of one-factor-at-a-time or classical design of experiments approaches but these techniques may not be efficient enough in preliminary screening phases.

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