Singular Genomics Systems has been granted a patent for efficient methods of preparing and sequencing multiple regions of a double-stranded polynucleotide. The method involves amplifying the polynucleotide by contacting it with an invasion primer and extending it with a polymerase. GlobalData’s report on Singular Genomics Systems gives a 360-degree view of the company including its patenting strategy. Buy the report here.

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According to GlobalData’s company profile on Singular Genomics Systems, DNA polymerase compositions was a key innovation area identified from patents. Singular Genomics Systems's grant share as of February 2024 was 29%. Grant share is based on the ratio of number of grants to total number of patents.

Method of preparing and sequencing double-stranded polynucleotide regions

Source: United States Patent and Trademark Office (USPTO). Credit: Singular Genomics Systems Inc

A recently granted patent (Publication Number: US11913071B2) discloses a method for amplifying double-stranded polynucleotides using an invasion primer. The method involves contacting the polynucleotide with the invasion primer, hybridizing the primer to the second strand, extending it with a polymerase to displace the first strand, and generating an amplification product by further extension with a polymerase. The process can be repeated through multiple invasion cycles, each potentially using different chemical denaturants or temperatures to enhance amplification efficiency. Prior amplification methods like bridge polymerase chain reaction (bPCR) can be employed before the invasion primer hybridization step to improve results.

Furthermore, the patent also covers a method for amplifying circular polynucleotides using a similar approach with additional steps involving multiple primers and extension products. The method includes hybridizing an invasion primer to the first extension product, displacing the second extension product, and generating immobilized amplification products. Sequencing of the amplification products can be performed by incorporating nucleotides into a sequencing primer with a polymerase and detecting them to identify each nucleotide in the extension strand. This innovative method offers a precise and efficient way to amplify polynucleotides for various applications, including sequencing and genetic analysis, by utilizing invasion primers and solid support for immobilization throughout the amplification process.

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GlobalData Patent Analytics tracks bibliographic data, legal events data, point in time patent ownerships, and backward and forward citations from global patenting offices. Textual analysis and official patent classifications are used to group patents into key thematic areas and link them to specific companies