C18 vs Normal Phase vs CPC: Which Method Fits Your Compound?
Selecting a preparative chromatography method should begin with the feed, not the instrument.
The target’s polarity and structure, its solubility, the impurities present, matrix load, required selectivity and production volume all influence whether C18 reversed-phase chromatography, normal-phase chromatography or centrifugal partition chromatography (CPC) is the most practical choice.