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Why Pressure Becomes a Scale-Up Problem in Preparative Chromatography

A C18 method may deliver excellent separation on an analytical HPLC—and still become impractical when production volume increases. The difficulty is not simply that a preparative column is larger. The problem is transferring a method operating at 100bar–300bar, or even higher in UHPLC, into equipment that must process substantially more material safely and economically.
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