Single Nucleotide Polymorphisms (SNP`s) have become an invaluable
tool in the field of Genetic Research. They are employed in a wide range
of scientific fields from Pharmacogenetics to animal breed identification
through to disease gene mapping. They are a popular choice of genetic
marker due to their ease of assay and analysis, however many standard
assays remain relatively expensive to use in high throughput.
In this application note we show the use of the PHERAstar multimode
plate reader for measurements in both SNP Genotyping chemistry
development and high throughput production genotyping. The new
KASPar™ system, developed by KBiosciences, was used to assess
the performance enhancements of the PHERAstar, using a new SNP
genotyping specific optical module with dual emission created by BMG
LABTECH. Using two photomultipliers allows for improved
accuracy and reduced read times by 30%.
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