Determining the protein concentration of samples is a necessary and
often used method in biochemistry. Different colorimetric protein
assays have been developed. The most commonly used methods are the
Bradford assay, the Lowry assay and the BCA assay. In this application
note we demonstrate how to determine the protein concentration of
samples by using the Bradford assay and the new FLUOstar Omega.
The Bradford assay is based on the binding of protein to a dye, leading to
a shift in the absorbance maximum of the dye1. After creating a standard
curve of protein solutions with known concentrations, the protein
concentration of unknown samples can be calculated. The dye used for
the Bradford assay is Coomassie® Brilliant Blue G-250.
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