Inositol-1,4,5-triphosphate (IP3) is an important second messenger in the cell. It is synthesized during cascade reactions that happen after stimulation of a G-protein coupled receptor (GPCR). Cisbio developed HTRF® terbium-based assays for the determination of IP1, a downstream metabolite of the unstable IP3. The assay was successfully performed on the PHERAstar and the PHERAstar Plus HTS microplate readers from BMG LABTECH.
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