One of the major bottlenecks in the production of biopharmaceuticals is the efficient expression of therapeutic proteins in microbial or mammalian cells.
The Escherichia coli pAVEway™ expression system described here has been developed to ensure high product titres and efficient scale up to GMP manufacture, whilst minimising many common issues seen in other expression systems, such as 'leaky' expression (expression of recombinant protein in the absence of inducer).
How it works
The use of a number of powerful E. coli RNA polymerase promoters, such as T7A3, λpL and tac, opens up a large host range in comparison with the popular T7 system that is limited to hosts carrying the λDE3 prophage.
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